{"product_id":"recombinant-human-ddr2-kinase-cd167b-protein-fc-tagactive-pksh031758","title":"Recombinant Human DDR2 Kinase\/CD167b Protein (Fc Tag)(Active) - PKSH031758","description":"\u003cp\u003eRecombinant Human DDR2 Kinase\/CD167b Protein (Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031758-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DDR2 Kinase; CD167b\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CD167; MIG20a; NTRKR3; TKT; TYRO10\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cardiovascular; Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-hFc\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_001014796.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2\/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2\/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2\/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2\/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Rat tail Collagen I at 10 μg\/ml can bind recombinant human DDR2-Fc Chimera with a linear range of 2. 5-80 ng\/ml. Scatchard analysis showed the affinity constant (Kd) of recombinant human DDR2-Fc Chimera bound to rat tail collagen I was 6. 8 nM.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Arg 399\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 69.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 87 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104325140659,"sku":"PKSH031758-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b73b6b98-a605-491f-a3bd-1f62eca9f70b.png?v=1709294336","url":"https:\/\/afsbio.com\/products\/recombinant-human-ddr2-kinase-cd167b-protein-fc-tagactive-pksh031758","provider":"AFSBio Inc.","version":"1.0","type":"link"}