{"product_id":"recombinant-human-dkk1-dkk-1-protein-his-tagactive-pksh031807","title":"Recombinant Human DKK1\/Dkk-1 Protein (His Tag)(Active) - PKSH031807","description":"\u003cp\u003eRecombinant Human DKK1\/Dkk-1 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH031807-20, PKSH031807-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DKK1; Dkk-1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Dickkopf-related protein 1; Dickkopf-1; Dkk-1; SK\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cardiovascular; Developmental Biology; immunology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_036374.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5\/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5\/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1\/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg\/ml in the presence of 10 ng\/mL of mouse Wnt3a.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 2-His 266\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 25.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43114024370355,"sku":"PKSH031807-20","price":424.2,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43114024403123,"sku":"PKSH031807-100","price":890.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_dbbdca5e-6361-4332-874c-ab589d228706.png?v=1709550405","url":"https:\/\/afsbio.com\/products\/recombinant-human-dkk1-dkk-1-protein-his-tagactive-pksh031807","provider":"AFSBio Inc.","version":"1.0","type":"link"}