{"product_id":"recombinant-human-dna-polymerase-β-polb-protein-his-tag-pksh032360","title":"Recombinant Human DNA Polymerase β\/POLB Protein (His Tag) - PKSH032360","description":"\u003cp\u003eRecombinant Human DNA Polymerase β\/POLB Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032360-10, PKSH032360-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DNA Polymerase β; POLB\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: DNA Polymerase Beta; POLB\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P06746\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: epigenetics and nuclear signal\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P06746\u003c\/p\u003e\n\n\u003cp\u003eBackground: Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser2-Glu335\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 39.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 39 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43092770226355,"sku":"PKSH032360-10","price":385.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43092770259123,"sku":"PKSH032360-50","price":735.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_dac7c17c-74dd-49af-aaaf-190ce95e8957.png?v=1709119628","url":"https:\/\/afsbio.com\/products\/recombinant-human-dna-polymerase-%ce%b2-polb-protein-his-tag-pksh032360","provider":"AFSBio Inc.","version":"1.0","type":"link"}