{"product_id":"recombinant-human-ece1-protein-his-tag-pksh033691","title":"Recombinant Human ECE1 Protein (His Tag) - PKSH033691","description":"\u003cp\u003eRecombinant Human ECE1 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033691-10, PKSH033691-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ECE1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Endothelin-converting enzyme 1; ECE-1\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cardiovascular; Cancer; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P42892\u003c\/p\u003e\n\n\u003cp\u003eAccession: P42892\u003c\/p\u003e\n\n\u003cp\u003eBackground: Endothelin-Converting Enzyme-1 (ECE-1) is a single-pass type I I transmembrane (TM) protein with a short cytoplasmic tail and a large ectodomain. ECE-1 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, and Kell, and several NEP-like proteins. It is widely expressed and has several alternatively spliced forms that differ in their TM domain or cytoplasmic tail. All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Endothelin-converting enzyme-1 is involved in the proteolytic processing of Endothelin-1 (EDN1), Endothelin-2 (EDN2), and Endothelin-3 (EDN3) to biologically active peptides. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction (HSCRCDAD). It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Gln90-Trp770\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 78.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 94-130 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43113982492851,"sku":"PKSH033691-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43113982525619,"sku":"PKSH033691-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a8125b1d-275c-45d5-9c6b-66c901b26cf3.png?v=1709549625","url":"https:\/\/afsbio.com\/products\/recombinant-human-ece1-protein-his-tag-pksh033691","provider":"AFSBio Inc.","version":"1.0","type":"link"}