{"product_id":"recombinant-human-egf-protein-active-pksh033687","title":"Recombinant Human EGF Protein (Active) - PKSH033687","description":"\u003cp\u003eRecombinant Human EGF Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 500μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH033687-500\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: EGF\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cell biology; Cancer;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P01133\u003c\/p\u003e\n\n\u003cp\u003eAccession: P01133\u003c\/p\u003e\n\n\u003cp\u003eBackground: Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Asn971-Arg1023\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from filtered solution of 1×PBS, pH 8.0 \u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 6.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 11 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107266855091,"sku":"PKSH033687-500","price":348.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_beccd085-25a6-40cc-9821-6a6d5bcfc3ee.png?v=1709382199","url":"https:\/\/afsbio.com\/products\/recombinant-human-egf-protein-active-pksh033687","provider":"AFSBio Inc.","version":"1.0","type":"link"}