{"product_id":"recombinant-human-egf-protein-rp01030","title":"Recombinant Human EGF Protein - RP01030","description":"\u003cp\u003eRecombinant Human EGF Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: RP01030-50, RP01030-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human EGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn 971-Arg 1023) of human EGF (Accession #NP_001954.2) fused with hFc, 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: EGF, HOMG4, URG\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P01133\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 1950\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin binding EGF like growth factor (HB_x005f\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its ability to stimulate EGF Receptor autophosphorylation in A431 cells.1-10 ng\/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.|2.Measured by its binding ability in a functional ELISA. Immobilized Human EGFR at 5 μg\/mL (100 μL\/well) can bind Human EGF with a linear range of 7-25 ng\/mL. 3.Measured in a cell proliferation assay using BALB\/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.065-0.26 ng\/mL, corresponding to a specific activity of 3.84×10^6 -1.54×10^7units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-hFc\u0026amp;His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"50ug","offer_id":45198361493683,"sku":"RP01030-50","price":182.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198361526451,"sku":"RP01030-100","price":168.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_db6124b2-c457-4f75-812e-e1347ef062c5.png?v=1766961182","url":"https:\/\/afsbio.com\/products\/recombinant-human-egf-protein-rp01030","provider":"AFSBio Inc.","version":"1.0","type":"link"}