{"product_id":"recombinant-human-egfr-erbb1-protein-aa-25-30gly-aa298-645-fc-tagactive-pksh032396","title":"Recombinant Human EGFR\/ErbB1 Protein (aa 25-30Gly\u0026aa298-645, Fc Tag)(Active) - PKSH032396","description":"\u003cp\u003eRecombinant Human EGFR\/ErbB1 Protein (aa 25-30Gly\u0026amp;aa298-645, Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032396-10, PKSH032396-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: EGFR; ErbB1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Epidermal growth factor receptor; EGFR; Proto-oncogene c-ErbB-1; Receptortyrosine-protein kinase erbB-1; EGFR\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cancer;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-Fc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P00533\u003c\/p\u003e\n\n\u003cp\u003eAccession: P00533\u003c\/p\u003e\n\n\u003cp\u003eBackground: The EGFR subfamily of receptor tyrosine kinases is composed of EGFR; ErbB2; ErbB3 and ErbB4. The EGFR shares 43% - 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2; resulting in cell signaling; heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF; amphiregulin; TGF-alpha; betacellulin; epiregulin; HB-EGF; epigen; and so on. Its signaling regulates multiple biological functions including cell proliferation; differentiation; motility; and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Human EGF(Cat: PKSH033687) at 10μg\/ml(100 μl\/well) can bind Human HER1-His.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Leu25-Val30Gly\u0026amp;Asn298-Ser645\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 65.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 90-120 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118137409715,"sku":"PKSH032396-10","price":180.6,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118137442483,"sku":"PKSH032396-50","price":525.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_325ba044-b3cb-40fa-a484-784890ef8712.png?v=1709636888","url":"https:\/\/afsbio.com\/products\/recombinant-human-egfr-erbb1-protein-aa-25-30gly-aa298-645-fc-tagactive-pksh032396","provider":"AFSBio Inc.","version":"1.0","type":"link"}