{"product_id":"recombinant-human-egfr-erbb1-protein-aa-25-645-fc-tagactive-pksh032398","title":"Recombinant Human EGFR\/ErbB1 Protein (aa 25-645, Fc Tag)(Active) - PKSH032398","description":"\u003cp\u003eRecombinant Human EGFR\/ErbB1 Protein (aa 25-645, Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032398-10, PKSH032398-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: EGFR; ErbB1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Epidermal growth factor receptor; EGFR; Proto-oncogene c-ErbB-1; Receptortyrosine-protein kinase erbB-1; EGFR\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cancer;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-Fc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P00533\u003c\/p\u003e\n\n\u003cp\u003eAccession: P00533\u003c\/p\u003e\n\n\u003cp\u003eBackground: EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK; PI3 kinase-AKT; PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16; activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Human EGF(Cat: PKSH033687) at 10μg\/ml(100 μl\/well) can bind Human EGFR-Fc.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Leu25-Ser645\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 95.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 130 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118137802931,"sku":"PKSH032398-10","price":163.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118137835699,"sku":"PKSH032398-50","price":415.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b2aca450-1d1c-4e68-b5fd-7bc7cac43901.png?v=1709636892","url":"https:\/\/afsbio.com\/products\/recombinant-human-egfr-erbb1-protein-aa-25-645-fc-tagactive-pksh032398","provider":"AFSBio Inc.","version":"1.0","type":"link"}