{"product_id":"recombinant-human-endothelial-monocyteaactivating-polypeptide-ii-rhuemap-ii-pr1015","title":"Recombinant Human Endothelial-MonocyteAActivating Polypeptide II (rHuEMAP-II) - PR1015","description":"\u003cp\u003eRecombinant Human Endothelial-MonocyteAActivating Polypeptide II (rHuEMAP-II)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR1015-5, PR1015-20\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5µg, 20µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 18.3 KDa, a single non-glycosylated polypeptide chain containing 166 amino acids.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;98% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-40 ng\/ml, corresponding to a Specific Activity of ＞2.5 x 104 IU\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP Rt VVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rHuEMAP-II as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: EMAP-II is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3 kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5µg","offer_id":42882224029875,"sku":"PR1015-5","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"20µg","offer_id":42882224062643,"sku":"PR1015-20","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_1768af69-94e0-4acc-a050-efca26aaa031.jpg?v=1702049584","url":"https:\/\/afsbio.com\/products\/recombinant-human-endothelial-monocyteaactivating-polypeptide-ii-rhuemap-ii-pr1015","provider":"AFSBio Inc.","version":"1.0","type":"link"}