{"product_id":"recombinant-human-ephrin-b1-efnb1-protein-his-fc-tagactive-pksh031370","title":"Recombinant Human Ephrin-B1\/EFNB1 Protein (His \u0026 Fc Tag)(Active) - PKSH031370","description":"\u003cp\u003eRecombinant Human Ephrin-B1\/EFNB1 Protein (His \u0026amp; Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031370-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Ephrin-B1; EFNB1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Ephrin-B1; EFL-3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; LERK-2; CFND; CFNS; EFB1; EFL3; Elk-L; EPLG2; LERK2\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cell biology; Cardiovascular; Neuroscience; Developmental Biology;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His \u0026amp; Fc\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_004420.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph\/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized mouse EphB3 at 2 μg\/ml (100 μl\/well) can bind human EFNB1 Fc chimera with a linear ranger of 1. 56-25 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Gly 232\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 51.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 64 and 36 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104309838003,"sku":"PKSH031370-100","price":789.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_00384b5c-26c6-48a7-919c-e1579a2cbbba.png?v=1709294001","url":"https:\/\/afsbio.com\/products\/recombinant-human-ephrin-b1-efnb1-protein-his-fc-tagactive-pksh031370","provider":"AFSBio Inc.","version":"1.0","type":"link"}