{"product_id":"recombinant-human-ezrin-ezr-protein-pksh033684","title":"Recombinant Human Ezrin\/EZR Protein - PKSH033684","description":"\u003cp\u003eRecombinant Human Ezrin\/EZR Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033684-10, PKSH033684-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Ezrin; EZR\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Ezrin; Cytovillin; Villin-2; p81; EZR; VIL2\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cancer;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P15311\u003c\/p\u003e\n\n\u003cp\u003eAccession: P15311\u003c\/p\u003e\n\n\u003cp\u003eBackground: Ezrin is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. The N-terminus of ezrin contains a FERM domain which is further subdivided into three subdomains. The C-terminus contain a ERM domain. As a member of the ERM protein family, Ezrin serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. Ezrin probably involved in connections of major cytoskeletal structures to the plasma membrane. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery). The C-terminal binds to actin, phosphatidylinositol bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2. In epithelial cells, Ezrin is required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, Ezrin is required for normal macropinocytosis.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-Leu586\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 10mM Hepes, pH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 69.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 80 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43113982132403,"sku":"PKSH033684-10","price":180.6,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43113982165171,"sku":"PKSH033684-50","price":525.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_e6167320-6c23-42c9-94dc-3384461cb153.png?v=1709549615","url":"https:\/\/afsbio.com\/products\/recombinant-human-ezrin-ezr-protein-pksh033684","provider":"AFSBio Inc.","version":"1.0","type":"link"}