{"product_id":"recombinant-human-fes-kinase-feline-sarcoma-oncogene-protein-his-gst-tagactive-pksh030338","title":"Recombinant Human FES Kinase\/Feline sarcoma oncogene Protein (His \u0026 GST Tag)(Active) - PKSH030338","description":"\u003cp\u003eRecombinant Human FES Kinase\/Feline sarcoma oncogene Protein (His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030338-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: FES Kinase\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: FPS\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P07332\u003c\/p\u003e\n\n\u003cp\u003eAccession: P07332-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Proto-oncogene tyrosine-protein kinase Fes\/Fps, also known as Proto-oncogene c-Fes, Proto-oncogene c-Fps, Feline sarcoma oncogene, FES and FPS, is a protein which contains oneFCH domain, oneprotein kinase domain and oneSH2 domain. FES is a non-receptor protein tyrosine kinase expressed in hematopoietic progenitors and differentiated myeloid cells. FES is observed in the nuclear, granular and plasma membrane fractions of primary human neutrophils and the myeloid leukemia cell line, HL-60. The nuclear localization is confirmed by immunocytochemistry of neutrophils. FES has been implicated in granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and erythropoietin signal transduction. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. FES is also involved in normal hematopoiesis. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 200 nmol\/min\/mg using Poly(Glu:Tyr) 4:1 as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Arg 822\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 75 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 121 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 110 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104347127987,"sku":"PKSH030338-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_46dc642b-4141-462f-9cd7-447b480a2572.png?v=1709294869","url":"https:\/\/afsbio.com\/products\/recombinant-human-fes-kinase-feline-sarcoma-oncogene-protein-his-gst-tagactive-pksh030338","provider":"AFSBio Inc.","version":"1.0","type":"link"}