{"product_id":"recombinant-human-fgf-1-fgfa-protein-active-pksh032431","title":"Recombinant Human FGF-1\/FGFa Protein (Active) - PKSH032431","description":"\u003cp\u003eRecombinant Human FGF-1\/FGFa Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032431-20, PKSH032431-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: FGF-1; FGFa\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Fibroblast Growth Factor 1; FGF-1; Acidic Fibroblast Growth Factor; aFGF; Endothelial Cell Growth Factor; ECGFHeparin-Binding Growth Factor 1; HBGF-1; FGF1; FGFA\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cardiovascular; Neuroscience; Cancer;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P05230\u003c\/p\u003e\n\n\u003cp\u003eAccession: P05230\u003c\/p\u003e\n\n\u003cp\u003eBackground: FGF acidic; also known as ECGF; FGF-1and HBGF-1; is a non-glycosylated heparin binding growth factor that is expressed in the brain; kidney; retina; smooth muscle cells; bone matrix; osteoblasts; astrocytes and endothelial cells. It is a mitogenic peptide that is produced by multiple cell types and stimulates the proliferation of cells of mesodermal; ectodermal; and endodermal origin. Its association with heparan sulfate is a prerequisite for activation of FGF receptors. Internalized FGF acidic migrates to the nucleus where it is phosphorylated by nuclear PKC delta; exported to the cytosol; dephosphorylated; and degraded. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using BALB\/c 3T3 cells. The ED50 for this effect is 0.2-2 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Phe16-Asp155\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 400mM NaCl, 1mM DTT, pH 8.0 .\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 15.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 16 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43118138228915,"sku":"PKSH032431-20","price":348.6,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43118138261683,"sku":"PKSH032431-100","price":1034.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_afb6096c-8605-40af-86f4-979c7901ecbe.png?v=1709636904","url":"https:\/\/afsbio.com\/products\/recombinant-human-fgf-1-fgfa-protein-active-pksh032431","provider":"AFSBio Inc.","version":"1.0","type":"link"}