{"product_id":"recombinant-human-fibronectin-fn-protein-active-pksh032450","title":"Recombinant Human Fibronectin\/FN Protein (Active) - PKSH032450","description":"\u003cp\u003eRecombinant Human Fibronectin\/FN Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH032450-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Fibronectin; FN\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Fibronectin; FN1; CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P02751\u003c\/p\u003e\n\n\u003cp\u003eAccession: P02751\u003c\/p\u003e\n\n\u003cp\u003eBackground: Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains; fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II; and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Pro1270-Ser1546\u0026amp;Ala1721-Thr2016\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 6.2.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 62.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 60 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107263643827,"sku":"PKSH032450-50","price":137.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_0215bac0-8c2d-45fc-bce8-28586d9fd508.png?v=1709382138","url":"https:\/\/afsbio.com\/products\/recombinant-human-fibronectin-fn-protein-active-pksh032450","provider":"AFSBio Inc.","version":"1.0","type":"link"}