{"product_id":"recombinant-human-flt3-ligand-flt3l-protein-rp00175","title":"Recombinant Human Flt3 ligand\/Flt3L Protein - RP00175","description":"\u003cp\u003eRecombinant Human Flt3 ligand\/Flt3L Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00175-10, RP00175-20, RP00175-50, RP00175-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Flt3 ligand\/Flt3L Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr27-Pro185) of human FLT3L\/Flt3 ligand\/FLT3LG (Accession #NP_001450.2) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: FL, FLT3L, FLT3LG, FLT3LG\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P49771\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 2323\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: FLT3LG, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors.FLT3LG is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Alternate splicing of human FLT3LG also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble FLT3LG signal through the tyrosine kinase receptor Flt-3\/Flk-2. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation.\u003c\/p\u003e \n\n\u003cp\u003eBio-Activity: 1、Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt3 ligand at 2 μg\/mL (100 μL\/well) can bind Recombinant Human Flt-3 with a linear range of 0.128-3.97 ng\/mL.2、Measured in a cell proliferation assay using Human OCI-AML5 cells. The ED50 for this effect is 0.09-0.36 ng\/mL, corresponding to a specific activity of 2.78×10^6 ~1.11×10^7 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: TQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLEATAPTAPQPP\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198308212915,"sku":"RP00175-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198308245683,"sku":"RP00175-20","price":266.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198308278451,"sku":"RP00175-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198308311219,"sku":"RP00175-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_e29be517-595d-4fef-96e2-0de215c74175.png?v=1766960426","url":"https:\/\/afsbio.com\/products\/recombinant-human-flt3-ligand-flt3l-protein-rp00175","provider":"AFSBio Inc.","version":"1.0","type":"link"}