{"product_id":"recombinant-human-frizzled-8-c-6his-avi-biotinylated-pksh034044","title":"Recombinant Human Frizzled-8 (C-6His-Avi) Biotinylated - PKSH034044","description":"\u003cp\u003eRecombinant Human Frizzled-8 (C-6His-Avi) Biotinylated\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH034044-20, PKSH034044-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Frizzled-8\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cancer; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Biotinylated\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His-Avi\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q9H461\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q9H461\u003c\/p\u003e\n\n\u003cp\u003eBackground: Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala28-Pro172\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 19.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 25-35 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43114016506035,"sku":"PKSH034044-20","price":478.8,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43114016538803,"sku":"PKSH034044-100","price":1293.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_208f29a0-0cd0-4e38-9574-bf7e6756aca4.png?v=1709550248","url":"https:\/\/afsbio.com\/products\/recombinant-human-frizzled-8-c-6his-avi-biotinylated-pksh034044","provider":"AFSBio Inc.","version":"1.0","type":"link"}