{"product_id":"recombinant-human-g-csfr-cd114-protein-active-pksh031747","title":"Recombinant Human G-CSFR\/CD114 Protein (Active) - PKSH031747","description":"\u003cp\u003eRecombinant Human G-CSFR\/CD114 Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031747-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: G-CSFR; CD114\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CD114; CSF3R; G-CSF R; GCSFR\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cancer; immunology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_000751.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine\/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this effect is typically 2-8 μg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Pro 621\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 69.0 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 94 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104324681907,"sku":"PKSH031747-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_48c06be6-d9cd-4209-a695-64cdc8385f5b.png?v=1709294327","url":"https:\/\/afsbio.com\/products\/recombinant-human-g-csfr-cd114-protein-active-pksh031747","provider":"AFSBio Inc.","version":"1.0","type":"link"}