{"product_id":"recombinant-human-gfer-protein-his-tag-pksh032412","title":"Recombinant Human GFER Protein (His Tag) - PKSH032412","description":"\u003cp\u003eRecombinant Human GFER Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032412-10, PKSH032412-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: GFER\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: FAD-linked sulfhydryl oxidase ALR; GFER; Augmenter of liver regeneration; hERV1; Hepatopoietin; GFER; ALR; HERV1; HPO\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P55789\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cell biology;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P55789\u003c\/p\u003e\n\n\u003cp\u003eBackground: GFER is a hepatotrophic growth factor and flavin-linked sulfhydryl oxidase which belongs to the Erv1\/ALR family of proteins. GFER is widely expressed in various human tissues. They are two isoforms of this protein. Isoform 1 could regenerate the redox-active disulfide bonds in CHCHD4\/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4\/MIA40 forms a transient intermolecular disulfide bridge with GFER\/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4\/MIA40, while GFER\/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. Isoform 2 may act as an autocrine hepatotrophic growth factor promoting liver regeneration. GFER could also induce the expression of S-adenosylmethionine decarboxyl-ase and ornithine decarboxylases (ODC). S-adenosylmethionine decarboxyl-ase and ornithine decarboxylases play an important role in the synthesis of polyamines.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Asp125\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 17.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43092776026291,"sku":"PKSH032412-10","price":163.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43092776059059,"sku":"PKSH032412-50","price":415.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_4d9c79ed-ce45-4f48-b788-8f8891cea40e.png?v=1709119741","url":"https:\/\/afsbio.com\/products\/recombinant-human-gfer-protein-his-tag-pksh032412","provider":"AFSBio Inc.","version":"1.0","type":"link"}