{"product_id":"recombinant-human-granzyme-b-ctla-1-gzmb-protein-rp02860","title":"Recombinant Human Granzyme B\/CTLA-1\/GZMB Protein - RP02860","description":"\u003cp\u003eRecombinant Human Granzyme B\/CTLA-1\/GZMB Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: RP02860-10, RP02860-20\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Granzyme B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly19-Tyr247) of human Granzyme B (Accession #NP_004122.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; GZMB\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P10144\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3002\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its ability to cleave a peptide substrate, tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl),in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB). The specific activity is \u0026gt;1500 pmol\/min\/μg. (The enzyme needs to be activated by Recombinant Mouse Active Cathepsin C)\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: GEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIRDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRY\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198350713011,"sku":"RP02860-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198350745779,"sku":"RP02860-20","price":308.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_82b59808-e61e-4f0f-af31-72daf72c1e4e.png?v=1766960924","url":"https:\/\/afsbio.com\/products\/recombinant-human-granzyme-b-ctla-1-gzmb-protein-rp02860","provider":"AFSBio Inc.","version":"1.0","type":"link"}