{"product_id":"recombinant-human-grk6-gprk6-protein-his-gst-tagactive-pksh030350","title":"Recombinant Human GRK6\/GPRK6 Protein (His \u0026 GST Tag)(Active) - PKSH030350","description":"\u003cp\u003eRecombinant Human GRK6\/GPRK6 Protein (His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030350-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: GRK6; GPRK6\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: GPRK6\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P43250\u003c\/p\u003e\n\n\u003cp\u003eAccession: P43250-2\u003c\/p\u003e\n\n\u003cp\u003eBackground: G protein-coupled receptor kinase 6, also known as G protein-coupled receptor kinase GRK6, GRK6 and GPRK6, is a lipid-anchor protein which belongs to theprotein kinase superfamily, AGC Ser\/Thr protein kinase family and GPRK subfamily. GRK6 \/ GPRK6 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. This protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described. GRK6 \/ GPRK6 is widely expressed. GRK6 \/ GPRK6 silencing causes suppression of signal transducer and activator of transcription 3 (STAT3) phosphorylation associated with reduction in MCL1 levels and phosphorylation, illustrating a potent mechanism for the cytotoxicity of GRK6 inhibition in multiple myeloma (MM) tumor cells. GRK6 also appears to be involved in responses to morphine. Inhibition of GRK6 represents a uniquely targeted novel therapeutic strategy in human multiple myeloma.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 7 nmol\/min\/mg using casein as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Arg 589\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 93 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 2mM GSH, 0.5mM PMSF, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 95.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 85 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104347947187,"sku":"PKSH030350-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_be5689ff-031c-4d05-9a43-163f6042f14d.png?v=1709294881","url":"https:\/\/afsbio.com\/products\/recombinant-human-grk6-gprk6-protein-his-gst-tagactive-pksh030350","provider":"AFSBio Inc.","version":"1.0","type":"link"}