{"product_id":"recombinant-human-hb-egf-protein-rp00754","title":"Recombinant Human HB-EGF Protein - RP00754","description":"\u003cp\u003eRecombinant Human HB-EGF Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00754-10, RP00754-20, RP00754-50, RP00754-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human HB-EGF Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Leu20-Leu148) of human HB-EGF (Accession #Q99075) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: DTR, DTS, DTSF, HEGFL, HBEGF, DTS, DTSF, HEGFL; HB-EGF\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q99075\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 1839\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Heparin-binding EGF-like growth factor (HB-EGF) is a 12-16 kDa member of the epidermal growth factor (EGF)family. It possesses an EGF-like domain, and a heparin-binding motif. Mature HB-EGF is a soluble peptide thatarises from proteolytic processing of the transmembrane form. Human HB -EGF shows 76% and 73% aasequence identity with rat and mouse HB-EGF, respectively. It is required for normal cardiac valve formationand normal heart function, promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation; it is mitogenic for fibroblasts, but not endothelial cells. HB-EGF classified as agroup 2 ErbB ligand based on its ability to activate both the EGF\/ErbB1 and ErbB4 receptors. Activityassociated with ErbB4 binding appears to be limited to non -mitogenic actions, while EGFR binding inducesboth mitogenic and non-mitogenic activity.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.15-0.60 ng\/mL, corresponding to a specific activity of 1.67×10^6 ~6.67×10^6 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: LVTGESLERLRRGLAAGTSNPDPPTVSTDQLLPLGGGRDRKVRDLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198312833203,"sku":"RP00754-10","price":182.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198312865971,"sku":"RP00754-20","price":217.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198312898739,"sku":"RP00754-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198312931507,"sku":"RP00754-100","price":420.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_455d8e51-0fb1-4363-aa8b-fb5dbfdb14f9.png?v=1766960477","url":"https:\/\/afsbio.com\/products\/recombinant-human-hb-egf-protein-rp00754","provider":"AFSBio Inc.","version":"1.0","type":"link"}