{"product_id":"recombinant-human-hgf-protein-his-tagactive-pksh032538","title":"Recombinant Human HGF Protein (His Tag)(Active) - PKSH032538","description":"\u003cp\u003eRecombinant Human HGF Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032538-10, PKSH032538-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: HGF\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cancer;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P14210\u003c\/p\u003e\n\n\u003cp\u003eAccession: P14210\u003c\/p\u003e\n\n\u003cp\u003eBackground: Hepatocyte growth factor\/scatter factor (HGF\/SF) is a paracrine cellular growth, motility and morphogenic factor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.3-1. 5 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Gln32-Ser728\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 26\u0026amp;53.7\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 32-38\u0026amp;55-65 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118141866163,"sku":"PKSH032538-10","price":180.6,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118141898931,"sku":"PKSH032538-50","price":525.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_919f2dee-0211-43ea-ba24-4e6b81efb814.png?v=1709636972","url":"https:\/\/afsbio.com\/products\/recombinant-human-hgf-protein-his-tagactive-pksh032538","provider":"AFSBio Inc.","version":"1.0","type":"link"}