{"product_id":"recombinant-human-ide-insulysin-protein-his-tag-pksh032593","title":"Recombinant Human IDE\/Insulysin Protein (His Tag) - PKSH032593","description":"\u003cp\u003eRecombinant Human IDE\/Insulysin Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032593-10, PKSH032593-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: IDE; Insulysin\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Insulin-Degrading Enzyme; Abeta-Degrading Protease; Insulin Protease; Insulinase; Insulysin; IDE\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P14735\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cell biology; Cardiovascular; Neuroscience; Cancer; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P14735\u003c\/p\u003e\n\n\u003cp\u003eBackground: Insulin-Degrading Enzyme (IDE) is a secreted enzyme that belongs to the peptidase M16 family. IDE is a large zinc-binding protease and cleaves multiple short polypeptides that vary considerably in sequence. IDE plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. IDE degrades amyloid formed by APP and IAPP. IDE may participate in the degradation and clearance of naturally secreted amyloid β-protein by neurons and microglia. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF α and β-endorphin.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met42-Leu1019\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05%Brij35, 10%Glycerol, pH7.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 114.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 120 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43068861415603,"sku":"PKSH032593-10","price":312.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43068861448371,"sku":"PKSH032593-50","price":830.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_f0402203-1847-453b-8a8a-92560daa554b.png?v=1708598285","url":"https:\/\/afsbio.com\/products\/recombinant-human-ide-insulysin-protein-his-tag-pksh032593","provider":"AFSBio Inc.","version":"1.0","type":"link"}