{"product_id":"recombinant-human-iduronate-2-sulfatase-ids-protein-his-tagactive-pksh031672","title":"Recombinant Human Iduronate 2-Sulfatase\/IDS Protein (His Tag)(Active) - PKSH031672","description":"\u003cp\u003eRecombinant Human Iduronate 2-Sulfatase\/IDS Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031672-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Iduronate 2-Sulfatase; IDS\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: IDS; MPS2; SIDS\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Tags \u0026amp; Cell Markers; Cell biology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_000193.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Iduronate 2-Sulfatase, also known as IDS, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. The human Iduronate 2-Sulfatase\/IDS consists of a signal peptide, a pro peptide and a mature chain that may be further processed into two chains. Among the identified 18 human sulfatases, Iduronate 2-Sulfatase\/IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG), heparan sulfate and dermatan sulfate. Multiple mutations in this X-chromosome localized gene result in Iduronate 2-Sulfatase\/IDS enzymatic deficiency, and lead to the sex-linked Mucopolysaccharidosis Type II (MPS II ), also known as Hunter Syndrome characterized by the lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe due to the level of Iduronate 2-Sulfatase\/IDS enzyme. Retroviral-mediated Iduronate 2-Sulfatase\/IDS gene transfer into lymphoid cells would be a promising gene therapeutic strategy.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is \u0026gt; 1.0 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Pro 550\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 87 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 61 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 85-95 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104320979123,"sku":"PKSH031672-50","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_09f4e2af-0fdc-40ef-b38d-902039e76abe.png?v=1709294248","url":"https:\/\/afsbio.com\/products\/recombinant-human-iduronate-2-sulfatase-ids-protein-his-tagactive-pksh031672","provider":"AFSBio Inc.","version":"1.0","type":"link"}