{"product_id":"recombinant-human-ifn-alpha-4-protein-rp01914","title":"Recombinant Human IFN-alpha 4 Protein - RP01914","description":"\u003cp\u003eRecombinant Human IFN-alpha 4 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20ug, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01914-20, RP01914-50, RP01914-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Recombinant Human IFN-alpha 4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Asp189) of Human IFN-alpha 4(Accession #NP_066546.1) fused with a His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Interferon alpha-4, IFN-alpha-4, Interferon alpha-4B, Interferon alpha-76, Interferon alpha-M1, IFNA4\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P05014\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3441\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: IFN-alpha 4,Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling. Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them. The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle. mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.26‑1.02 ng\/mL, corresponding to a specific activity of 9.80×10^5 ~3.85×10^6 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: CDLPQTHSLGNRRALILLAQMGRISHFSCLKDRHDFGFPEEEFDGHQFQKAQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNDLEACVIQEVGVEETPLMNEDSILAVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"20ug","offer_id":45198385479859,"sku":"RP01914-20","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198385512627,"sku":"RP01914-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198385545395,"sku":"RP01914-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_d068aee9-e713-407d-ad31-11cf3f5db980.png?v=1766961537","url":"https:\/\/afsbio.com\/products\/recombinant-human-ifn-alpha-4-protein-rp01914","provider":"AFSBio Inc.","version":"1.0","type":"link"}