{"product_id":"recombinant-human-ifn-gamma-protein-rp01038","title":"Recombinant Human IFN-gamma Protein - RP01038","description":"\u003cp\u003eRecombinant Human IFN-gamma Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20ug, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01038-20, RP01038-50, RP01038-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human IFN-gamma Protein is produced by E. coli expression system. The target protein is expressed with sequence ( Gln24-Gln166) of human Interferon Gamma (Accession #NP_000610.2).\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IFNG, IFG, IFI\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P01579-1\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3458\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interferon-gamma (IFN-gamma ) is a secreted protein which belongs to the type II interferon family.Recombinant Human IFN-gamma is a 16.8 kDa protein containing 143 amino acid residues. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. Interferon-gamma is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1\/CD119 at 1 μg\/mL (100 μL\/well) can bind Human IFNG with a linear range of 0.98-1.97 ng\/mL.|2.Measured in a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 0.74-2.96 ng\/mL, corresponding to a specific activity of 3.38×10^5 ~1.35×10^6 units\/mg. 3.Flow cytometry: 1X10^6 A549 cells treated with IFN-γ (RP01038,1 ng\/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl\/Test,left) or PE Rabbit anti-Human PD-L1\/CD274 mAb (A26692,5 μl\/Test,right).|4.Flow cytometry: 1X10^6 NCI-H460 cells treated with IFN-γ (RP01038,1 ng\/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl\/Test,left) or PE Rabbit anti-Human PD-L1\/CD274 mAb (A26692,5 μl\/Test,right)\u003c\/p\u003e\n\n\u003cp\u003eSource: E. coli\u003c\/p\u003e\n\n\u003cp\u003eTag: No tag\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"20ug","offer_id":45198384693427,"sku":"RP01038-20","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198384726195,"sku":"RP01038-50","price":168.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198384758963,"sku":"RP01038-100","price":280.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_a4920a23-e141-4fac-add0-068aa5607ac6.png?v=1766961525","url":"https:\/\/afsbio.com\/products\/recombinant-human-ifn-gamma-protein-rp01038","provider":"AFSBio Inc.","version":"1.0","type":"link"}