{"product_id":"recombinant-human-igf-i-protein-rp00996","title":"Recombinant Human IGF-I Protein - RP00996","description":"\u003cp\u003eRecombinant Human IGF-I Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00996-10, RP00996-20, RP00996-50, RP00996-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human IGF-I Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ( Gly 49 - Ala 118) of human IGF1 (Accession #NP_001104755.1) fused with an Fc, 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IGF1, IGF-I, IGFI, MGF, IGFI, MGF\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P05019-1\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3479\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 85 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells. 0.01-1 ng\/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.|2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 μg\/mL (100 μL\/well) can bind recombinant human IGF1 with a linear range of 30-250 ng\/mL. 3.Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 7.5-30 ng\/mL, corresponding to a specific activity of 3.33×10^4 -1.33×10^5 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-hFc\u0026amp;His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198284128435,"sku":"RP00996-10","price":140.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198284161203,"sku":"RP00996-20","price":154.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198284193971,"sku":"RP00996-50","price":168.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198284226739,"sku":"RP00996-100","price":280.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_5f1b0538-1938-4df0-b6b4-5cbb64a0134d.png?v=1766960116","url":"https:\/\/afsbio.com\/products\/recombinant-human-igf-i-protein-rp00996","provider":"AFSBio Inc.","version":"1.0","type":"link"}