{"product_id":"recombinant-human-il-12-il-12a-il-12b-protein-rp01232","title":"Recombinant Human IL-12\/IL-12A\u0026IL-12B Protein - RP01232","description":"\u003cp\u003eRecombinant Human IL-12\/IL-12A\u0026amp;IL-12B Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01232-10, RP01232-20, RP01232-50, RP01232-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Active Recombinant Human IL-12A\u0026amp;IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328\u0026amp;Arg23-Ser219) of human IL-12 (Accession #NP_002178.2\/NP_000873.2) fused with a 6×His tag at the C-terminus(IL12B),Flag tag at the C-terminus(IL12A).\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CLMF, IL12, p70, Interleukin-12, IL12A, P35, NKSF\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P29459\u0026amp;P29460\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3592\u0026amp;3593\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL-12 at 1 μg\/mL (100 μL\/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng\/mL.|2.Measured in a cell proliferation assay using anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat). The ED50 for this effect is typically 0.1-0.4 ng\/mL, corresponding to a specific activity of 2.5×10^6 -1.0×10^7units\/mg.|3.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-8 ng\/mL, corresponding to a specific activity of 1.25×10^5 ~5×10^5 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His(IL12B)\u0026amp;C-Flag(IL12A)\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS\/\/RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198290976947,"sku":"RP01232-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198291009715,"sku":"RP01232-20","price":308.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198291042483,"sku":"RP01232-50","price":490.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198291075251,"sku":"RP01232-100","price":784.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_03b05b1d-8525-44e4-ba7c-55e196644fb3.png?v=1766960199","url":"https:\/\/afsbio.com\/products\/recombinant-human-il-12-il-12a-il-12b-protein-rp01232","provider":"AFSBio Inc.","version":"1.0","type":"link"}