{"product_id":"recombinant-human-il-2-aldesleukin-ept147","title":"Recombinant Human IL-2 (aldesleukin) - EPT147","description":"\u003cp\u003eRecombinant Human IL-2 (aldesleukin)\u003c\/p\u003e\n\n\u003cp\u003eSize: 10ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: EPT147-10\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15, 300 Daltons. The chemical name is des-alanyl-1, serine-145 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: it is not glycosylated; the molecule has serine substituted for cysteine at amino acid position 145; the aggregation state of molecule is likely to be different from that of native IL-2.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Pro22-Thr153 (Cys145Ser) is expressed.\u003c\/p\u003e\n\n\u003cp\u003eAccession: P60568\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 15.5 KDa\u003c\/p\u003e\n\n\u003cp\u003eApparent Molecular Weight: 14 KDa, reducing conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage Condition: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003ePurity: Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eRedissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg\/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\n\n\u003cp\u003eDelivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"Default Title","offer_id":43211033215155,"sku":"EPT147-10","price":138.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_8e3c273e-093c-475a-ab89-7ab0120b36d2.png?v=1767117872","url":"https:\/\/afsbio.com\/products\/recombinant-human-il-2-aldesleukin-ept147","provider":"AFSBio Inc.","version":"1.0","type":"link"}