{"product_id":"recombinant-human-il-23il23a-il12b-heterodimer-protein-his-tagactive-pksh030445","title":"Recombinant Human IL-23(IL23A\u0026IL12B Heterodimer) Protein (His Tag)(Active) - PKSH030445","description":"\u003cp\u003eRecombinant Human IL-23(IL23A\u0026amp;IL12B Heterodimer) Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH030445-5, PKSH030445-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: IL-23\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2; IL-23; IL-23A; IL23P19; P19\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Microbiology; Cancer; immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture; ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q9NPF7\u0026amp;NP_002178.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.\u003c\/p\u003e\n\n\u003cp\u003eActivity: 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. \u003cbr\u003e\n2. Immobilized human IL23A-His+IL12B-His at 10 μg\/ml (100 μl\/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg\/ml. \u003cbr\u003e\n3. Immobilized human IL23A-His+IL12B-His at 10 μg\/ml (100 μl\/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg\/ml. \u003cbr\u003e\n4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng\/mL.\u003c\/p\u003e \n\n\u003cp\u003eSequence: Met 1-Pro189\u0026amp;Met 1-Ser 28\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 56.3 (20.1 + 36.2) kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 22\u0026amp;45 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"5μg","offer_id":43114022404275,"sku":"PKSH030445-5","price":177.8,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43114022437043,"sku":"PKSH030445-100","price":1540.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b144d6e3-19b3-441e-b558-8c5e3e3bd89a.png?v=1709550367","url":"https:\/\/afsbio.com\/products\/recombinant-human-il-23il23a-il12b-heterodimer-protein-his-tagactive-pksh030445","provider":"AFSBio Inc.","version":"1.0","type":"link"}