{"product_id":"recombinant-human-il-25-il-17e-protein-rp01755","title":"Recombinant human IL-25\/IL-17E Protein - RP01755","description":"\u003cp\u003eRecombinant human IL-25\/IL-17E Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20ug, 50ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01755-10, RP01755-20, RP01755-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant human IL-25\/IL-17E Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr33-Gly177) of human IL-25(Accession #NP_073626.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IL17E; IL-25\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q9H293\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 64806\u003c\/p\u003e\n\n\u003cp\u003eSpecies: human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its ability to induce CXCL1\/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 13.32-53.24 ng\/mL, corresponding to a specific activity of 1.88×10^4 ~7.51×10^4 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of 20 mM Tris,150 mM NaCl,1 mM EDTA,pH8.0\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: YSHWPSCCPSKGQDTSEELLRWSTVPVPPLEPARPNRHPESCRASEDGPLNSRAISPWRYELDRDLNRLPQDLYHARCLCPHCVSLQTGSHMDPRGNSELLYHNQTVFYRRPCHGEKGTHKGYCLERRLYRVSLACVCVRPRVMG\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198368702643,"sku":"RP01755-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198368735411,"sku":"RP01755-20","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198368768179,"sku":"RP01755-50","price":350.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_a160b780-e412-44b9-8db8-a08664781624.png?v=1766961290","url":"https:\/\/afsbio.com\/products\/recombinant-human-il-25-il-17e-protein-rp01755","provider":"AFSBio Inc.","version":"1.0","type":"link"}