{"product_id":"recombinant-human-il-3-protein-rp01362","title":"Recombinant Human IL-3 Protein - RP01362","description":"\u003cp\u003eRecombinant Human IL-3 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01362-10, RP01362-20, RP01362-50, RP01362-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human IL-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala20-Phe152) of human interleukin-3\/IL-3 (Accession #NP_000579.2) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IL3, IL-3, MCGF, MULTI-CSF\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P08700\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3562\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3\/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3\/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3\/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1.5-6 ng\/mL.|2.Recombinant Human TPO(50 ng\/mL, Cat. RP00174) , IL-3(15 ng\/mL), IL-6(15 ng\/mL, Cat. RP00201) and IL-11(15 ng\/mL, Cat. RP00050) induce hematopoietic stem and progenitor cells to differentiate into megakaryocytes. After 6 days, the induction of CD41\/42+ megakaryocytes was successful.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198321680563,"sku":"RP01362-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198321713331,"sku":"RP01362-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198321746099,"sku":"RP01362-50","price":315.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198321778867,"sku":"RP01362-100","price":504.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_7a4247b6-1f0a-4837-bc48-3ceec79c0c4f.png?v=1766960586","url":"https:\/\/afsbio.com\/products\/recombinant-human-il-3-protein-rp01362","provider":"AFSBio Inc.","version":"1.0","type":"link"}