{"product_id":"recombinant-human-il-4-c-6his-ept247","title":"Recombinant Human IL-4 (C-6His) - EPT247","description":"\u003cp\u003eRecombinant Human IL-4 (C-6His)\u003c\/p\u003e\n\n\u003cp\u003eSize: 10ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: EPT247-10\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed with a 6His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAccession: P05112\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 16 KDa\u003c\/p\u003e\n\n\u003cp\u003eApparent Molecular Weight: 18 KDa, reducing conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage Condition: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003ePurity: Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Loaded Cynomolgus IL-4RA-Fc (Cat#C939) on Protein A Biosensor, can bind Human IL-4-His (Cat#CX88) with an affinity constant of 0.33 nM as determined in BLI assay. \u003c\/p\u003e\n\n\u003cp\u003eRedissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg\/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\n\n\u003cp\u003eDelivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"Default Title","offer_id":43211048779955,"sku":"EPT247-10","price":278.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_0f4d1b52-9a35-4447-ba77-6c12df2b26b4.png?v=1767117911","url":"https:\/\/afsbio.com\/products\/recombinant-human-il-4-c-6his-ept247","provider":"AFSBio Inc.","version":"1.0","type":"link"}