{"product_id":"recombinant-human-insulin-protein-active-pksh032458","title":"Recombinant Human Insulin Protein (Active) - PKSH032458","description":"\u003cp\u003eRecombinant Human Insulin Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH032458-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Insulin\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Yeast\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P01308\u003c\/p\u003e\n\n\u003cp\u003eAccession: P01308\u003c\/p\u003e\n\n\u003cp\u003eBackground: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.2-1 µg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Phe1-Lys29\u0026amp;Gly1-Asn21\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 6 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 6 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107263742131,"sku":"PKSH032458-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_3c24e2ec-a8d9-4644-8ab4-801bb23c4fa8.png?v=1709382140","url":"https:\/\/afsbio.com\/products\/recombinant-human-insulin-protein-active-pksh032458","provider":"AFSBio Inc.","version":"1.0","type":"link"}