{"product_id":"recombinant-human-interleukin-1-receptor-antagonist-rhu-il-1ra-pr1051","title":"Recombinant Human Interleukin-1 receptor antagonist (rHu IL-1ra) - PR1051","description":"\u003cp\u003eRecombinant Human Interleukin-1 receptor antagonist (rHu IL-1ra)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR1051-20, PR1051-100, PR1051-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20µg, 100µg, 1.0mg (Contact us for the 1.0mg option)\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight:Approximately 17.0 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng\/ml, corresponding to a Specific Activity of ＞2.0 x 106 IU\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVVPIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE\u003c\/p\u003e \n\n\u003cp\u003eEndotoxin: Less than 1EU\/g of rHuIL-1ra as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.   Made  in China\u003c\/p\u003e\n\n\u003cp\u003eInterleukin-1 receptor antagonist (IL-1ra) is a member of the IL-1 family. Endogenous IL-1ra is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra.The regulated expression of IL1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1 , TNF-alpha , or PDGF.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"20µg","offer_id":42880591691955,"sku":"PR1051-20","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µg","offer_id":42880591724723,"sku":"PR1051-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f29b585e-98d9-4b67-9317-765220b4e5c9.jpg?v=1701982012","url":"https:\/\/afsbio.com\/products\/recombinant-human-interleukin-1-receptor-antagonist-rhu-il-1ra-pr1051","provider":"AFSBio Inc.","version":"1.0","type":"link"}