{"product_id":"recombinant-human-interleukin-15-rhuil-15-pr1062","title":"Recombinant Human Interleukin-15 (rHuIL-15) - PR1062","description":"\u003cp\u003eRecombinant Human Interleukin-15 (rHuIL-15)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR1062-2, PR1062-10\u003c\/p\u003e\n\n\u003cp\u003eSizes: 2µg, 10µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;96% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be \u0026lt; 0.5 ng\/ml., corresponding to a specific activity of \u0026gt; 2 x 106 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDt VENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rHuIL-15 as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: Interleukin 15 is a recently identified novel cytokine that shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. cDNA clones encoding this cytokine have been isolated from simian, murine and human cell sources. Human IL-15 shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. Human IL-15 cDNA encodes a 162 amino acid residue precursor protein containing a 48 amino acid residue signal peptide that is cleaved to yield the 114 amino acid residue mature protein. The IL-15 mRNA is expressed by a wide variety of cells and tissues and is most abundantly expressed by adherent peripheral blood mononuclear cells, fibroblasts and epithelial cells. However, in activated peripheral blood T lymphocytes where IL-2 is highly expressed, the expression of IL-15 is not detectable. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone. (Grabstein, K. et al., 1994, Science 264:965; Giri, J. et al., 1994, EMBO J. 13:2822).\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"2µg","offer_id":42882218950835,"sku":"PR1062-2","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"10µg","offer_id":42882218983603,"sku":"PR1062-10","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_03e8d509-9c50-4851-b5bd-1d5584830cc2.jpg?v=1702049469","url":"https:\/\/afsbio.com\/products\/recombinant-human-interleukin-15-rhuil-15-pr1062","provider":"AFSBio Inc.","version":"1.0","type":"link"}