{"product_id":"recombinant-human-interleukin-7-il-7-protein-his-tagactive-pksh032654","title":"Recombinant Human Interleukin-7\/IL-7 Protein (His Tag)(Active) - PKSH032654","description":"\u003cp\u003eRecombinant Human Interleukin-7\/IL-7 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032654-10, PKSH032654-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Interleukin-7; IL-7\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Interleukin-7; IL-7; IL7\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P13232\u003c\/p\u003e\n\n\u003cp\u003eAccession: P13232\u003c\/p\u003e\n\n\u003cp\u003eBackground: Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK\/STAT and PI3K\/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 50-300 pg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Asp26-His177\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 18.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 25 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118148255923,"sku":"PKSH032654-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118148288691,"sku":"PKSH032654-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a51a6373-5a0d-42b6-b695-ccc85687a59f.png?v=1709637086","url":"https:\/\/afsbio.com\/products\/recombinant-human-interleukin-7-il-7-protein-his-tagactive-pksh032654","provider":"AFSBio Inc.","version":"1.0","type":"link"}