{"product_id":"recombinant-human-keap1-inrf2-protein-his-gst-avi-tag-pksh030944","title":"Recombinant Human KEAP1\/INRF2 Protein (His \u0026 GST \u0026 AVI Tag) - PKSH030944","description":"\u003cp\u003eRecombinant Human KEAP1\/INRF2 Protein (His \u0026amp; GST \u0026amp; AVI Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH030944-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: KEAP1; INRF2\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: INRF2; KEAP-1; KLHL19\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q14145\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: epigenetics and nuclear signal\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Biotinylated\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST \u0026amp; AVI\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q14145\u003c\/p\u003e\n\n\u003cp\u003eBackground: Kelch-like ECH-associated protein 1; also known as cytosolic inhibitor of Nrf2; Kelch-like protein 19; KEAP1 and INRF2; is a cytoplasm and nucleus protein which contains one BACK (BTB\/Kelch associated) domain; one BTB (POZ) domain and six Kelch repeats. KEAP1 \/ INRF2 is broadly expressed; with highest levels in skeletal muscle. KEAP1 \/ INRF2 is a key regulator of the NRF2 transcription factor; which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions; KEAP1 \/ INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 \/ INRF2 retains NFE2L2 \/ NRF2 in the cytosol. KEAP1 \/ INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 \/ NRF2 for ubiquitination and degradation by the proteasome; thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 \/ INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Gln 2-Cys 624\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 99.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 85 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43083645288627,"sku":"PKSH030944-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_9eca2efc-9bbf-407b-8f92-eae74d719ced.png?v=1708946030","url":"https:\/\/afsbio.com\/products\/recombinant-human-keap1-inrf2-protein-his-gst-avi-tag-pksh030944","provider":"AFSBio Inc.","version":"1.0","type":"link"}