{"product_id":"recombinant-human-klotho-beta-klb-protein-rp02210","title":"Recombinant Human Klotho beta\/KLB Protein - RP02210","description":"\u003cp\u003eRecombinant Human Klotho beta\/KLB Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: RP02210-10, RP02210-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Klotho beta\/KLB Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met30-Thr983) of Human Klotho beta\/KLB (Accession #NP_783864.1) fused with a his tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: KLB, Beta-klotho, BKL, BetaKlotho, Klotho beta-like protein\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q86Z14\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 152831\u003c\/p\u003e\n\n\u003cp\u003eSpecies: human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Klotho beta\/KLB iis a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes. However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Immobilized Human Klotho at 1 μg\/mL(100 μL\/Well).Dose response curve for Anti-Klotho Ab.1 with the EC50 of 0.4 μg\/ml determined by ELISA.|Immobilized Human Klotho at 2μg\/ml(100 μL\/Well).Dose response curve for Anti-Klotho Ab. 2 with the EC50 of 20.5 ng\/mL determined by ELISA.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: MSNGGLQRSVILSALILLRAVTGFSGDGRAIWSKNPNFTPVNESQLFLYDTFPKNFFWGIGTGALQVEGSWKKDGKGPSIWDHFIHTHLKNVSSTNGSSDSYIFLEKDLSALDFIGVSFYQFSISWPRLFPDGIVTVANAKGLQYYSTLLDALVLRNIEPIVTLYHWDLPLALQEKYGGWKNDTIIDIFNDYATYCFQMFGDRVKYWITIHNPYLVAWHGYGTGMHAPGEKGNLAAVYTVGHNLIKAHSKVWHNYNTHFRPHQKGWLSITLGSHWIEPNRSENTMDIFKCQQSMVSVLGWFANPIHGDGDYPEGMRKKLFSVLPIFSEAEKHEMRGTADFFAFSFGPNNFKPLNTMAKMGQNVSLNLREALNWIKLEYNNPRILIAENGWFTDSRVKTEDTTAIYMMKNFLSQVLQAIRLDEIRVFGYTAWSLLDGFEWQDAYTIRRGLFYVDFNSKQKERKPKSSAHYYKQIIRENGFSLKESTPDVQGQFPCDFSWGVTESVLKPESVASSPQFSDPHLYVWNATGNRLLHRVEGVRLKTRPAQCTDFVNIKKQLEMLARMKVTHYRFALDWASVLPTGNLSAVNRQALRYYRCVVSEGLKLGISAMVTLYYPTHAHLGLPEPLLHADGWLNPSTAEAFQAYAGLCFQELGDLVKLWITINEPNRLSDIYNRSGNDTYGAAHNLLVAHALAWRLYDRQFRPSQRGAVSLSLHADWAEPANPYADSHWRAAERFLQFEIAWFAEPLFKTGDYPAAMREYIASKHRRGLSSSALPRLTEAERRLLKGTVDFCALNHFTTRFVMHEQLAGSRYDSDRDIQFLQDITRLSSPTRLAVIPWGVRKLLRWVRRNYGDMDIYITASGIDDQALEDDRLRKYYLGKYLQEVLKAYLIDKVRIKGYYAFKLAEEKSKPRFGFFTSDFKAKSSIQFYNKVISSRGFPFENSSSRCSQTQENT\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198359068851,"sku":"RP02210-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198359101619,"sku":"RP02210-100","price":784.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_0288a062-d645-48f9-b23d-167ae0cc40a8.png?v=1766961128","url":"https:\/\/afsbio.com\/products\/recombinant-human-klotho-beta-klb-protein-rp02210","provider":"AFSBio Inc.","version":"1.0","type":"link"}