{"product_id":"recombinant-human-legumain-lgmn-protein-his-tag-pksh032687","title":"Recombinant Human Legumain\/LGMN Protein (His Tag) - PKSH032687","description":"\u003cp\u003eRecombinant Human Legumain\/LGMN Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032687-10, PKSH032687-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Legumain; LGMN\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Legumain; Asparaginyl Endopeptidase; Protease Cysteine 1; LGMN; PRSC1\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q99538\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cell biology; Cancer; immunology; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q99538\u003c\/p\u003e\n\n\u003cp\u003eBackground: Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal\/endosomal system and negative regulation of neuron apoptosis.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ile18-Tyr433\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 48.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 56 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43068876488883,"sku":"PKSH032687-10","price":312.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43068876521651,"sku":"PKSH032687-50","price":830.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a2d5e7af-1405-49a9-92e6-4217b34d0f06.png?v=1708598451","url":"https:\/\/afsbio.com\/products\/recombinant-human-legumain-lgmn-protein-his-tag-pksh032687","provider":"AFSBio Inc.","version":"1.0","type":"link"}