{"product_id":"recombinant-human-leukemia-inhibitory-factor-lif-protein-rp00089","title":"Recombinant Human Leukemia inhibitory factor\/LIF Protein - RP00089","description":"\u003cp\u003eRecombinant Human Leukemia inhibitory factor\/LIF Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00089-10, RP00089-20, RP00089-50, RP00089-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Leukemia inhibitory factor\/LIF Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser23-Phe202) of human LIF (Accession #NP_002300.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: LIF, CDF, DIA, HILDA, MLPLI\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P15018\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3976\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.\u003c\/p\u003e \n\n\u003cp\u003eBio-Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng\/mL, corresponding to a specific activity of 3.33×10^6 -1×10^7units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198294122675,"sku":"RP00089-10","price":182.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198294155443,"sku":"RP00089-20","price":217.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198294188211,"sku":"RP00089-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198294220979,"sku":"RP00089-100","price":420.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_d1183784-cf7b-4e33-a344-810cc8bfb38f.png?v=1766960235","url":"https:\/\/afsbio.com\/products\/recombinant-human-leukemia-inhibitory-factor-lif-protein-rp00089","provider":"AFSBio Inc.","version":"1.0","type":"link"}