{"product_id":"recombinant-human-lilrb4-cd85k-ilt3-protein-his-tag-pksh033603","title":"Recombinant Human LILRB4\/CD85k\/ILT3 Protein (His Tag) - PKSH033603","description":"\u003cp\u003eRecombinant Human LILRB4\/CD85k\/ILT3 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033603-10, PKSH033603-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: LILRB4; CD85k; ILT3\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Leukocyte immunoglobulin-like receptor subfamily B member 4; Mast cell surface glycoprotein Gp49B; CD85k; Lilrb4; Gp49b\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cardiovascular; epigenetics and nuclear signal; immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q8NHJ6\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q8NHJ6\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4\/CD85k\/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains; a 22 aa transmembrane segment; and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM). Within the ECD; mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4; respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells (DC); monocytes; macrophages; and vascular endothelial cells (EC). Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling; leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn; CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes; DC; and EC.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Gln22-Glu259\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 27.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 37 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43113974857907,"sku":"PKSH033603-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43113974890675,"sku":"PKSH033603-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_02a946c0-70a8-4ca0-8777-eb3085c9f43d.png?v=1709549452","url":"https:\/\/afsbio.com\/products\/recombinant-human-lilrb4-cd85k-ilt3-protein-his-tag-pksh033603","provider":"AFSBio Inc.","version":"1.0","type":"link"}