{"product_id":"recombinant-human-lipocalin-2-ngal-lcn2-protein-rp01955","title":"Recombinant Human Lipocalin-2\/NGAL\/LCN2 Protein - RP01955","description":"\u003cp\u003eRecombinant Human Lipocalin-2\/NGAL\/LCN2 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01955-10, RP01955-20, RP01955-50, RP01955-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Lipocalin-2\/NGAL\/LCN2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln21-Gly198)of Human Lipocalin-2\/NGAL\/LCN2 (Accession #NP_005555.2) fused with a His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: LCN2, HNL, NGAL, Neutrophil gelatinase-associated lipocalin, NGAL, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P80188\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3934\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant. The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron. Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1、Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant mouse Lipocalin-2. Recombinant human Lipocalin-2 can bind \u0026gt;0.60 μM of Fe(DHBA)3.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: QDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQKMYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAMVFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDG\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU\/μg of the protein by LAL method\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198340849843,"sku":"RP01955-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198340882611,"sku":"RP01955-20","price":266.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198340915379,"sku":"RP01955-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198340948147,"sku":"RP01955-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_0af78fc6-3af5-4eea-9cda-4ae58227c1de.png?v=1766960801","url":"https:\/\/afsbio.com\/products\/recombinant-human-lipocalin-2-ngal-lcn2-protein-rp01955","provider":"AFSBio Inc.","version":"1.0","type":"link"}