{"product_id":"recombinant-human-lr3-igf-1e51r-protein-rp00825","title":"Recombinant Human LR3-IGF-1(E51R) Protein - RP00825","description":"\u003cp\u003eRecombinant Human LR3-IGF-1(E51R) Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: RP00825-50, RP00825-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human LR3-IGF-1(E51R) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Gly49-Ala118) of human IGF-1 (Accession #P05019) with the substitution of an Arg for Glu at position three and the addition of a thirteen aa N-terminal extension.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IGF1, IBP1, IGF-1, Insulin-like growth factor 1, Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P05019\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 3479\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Long R3 IGF-1 (LR3 IGF-1) is a synthetic analog of IGF-1 that is generated by modifying the AA sequence for mature human IGF-1. These modifications include the substitution of an Arg for Glu at position three of the mature IGF-1 sequence and the addition of a thirteen aa N-terminal extension, which is derived from methionyl porcine Growth Hormone. These aa changes generate a protein that is still capable of binding to IGF-1 and Insulin receptors, but shows considerably lower affinity binding to IGFBPs compared to wild-type IGF-1. As a result, LR3 IGF-1 has an increased half-life and displays increased biological potency compared to IGF-1.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1、Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is 9.85-39.4 ng\/mL, corresponding to a specific activity of 2.54×10^4 ~1.01×10^5 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: E. coli\u003c\/p\u003e\n\n\u003cp\u003eTag: NO-Tag\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: MFPAMPLSSLFVNGPRTLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"50ug","offer_id":45198361886899,"sku":"RP00825-50","price":168.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198361919667,"sku":"RP00825-100","price":210.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_46236487-b0f7-4fa0-84d9-e6ff554f1bba.png?v=1766961190","url":"https:\/\/afsbio.com\/products\/recombinant-human-lr3-igf-1e51r-protein-rp00825","provider":"AFSBio Inc.","version":"1.0","type":"link"}