{"product_id":"recombinant-human-lrap-erap2-protein-his-tagactive-pksh031454","title":"Recombinant Human LRAP\/ERAP2 Protein (His Tag)(Active) - PKSH031454","description":"\u003cp\u003eRecombinant Human LRAP\/ERAP2 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031454-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: LRAP; ERAP2\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: FLJ23633; FLJ23701; FLJ23807; L-RAP; LRAP; LRAP.ERAP2\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cell biology; immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_071745.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 960-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH(X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS\/ARTS1\/ERAP1 and LNPEP\/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is \u0026gt; 50 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala 56-Thr 960\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 106 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 115-125 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104312557747,"sku":"PKSH031454-50","price":1213.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_28b20a9c-2e81-43ed-9849-7e765052133f.png?v=1709294071","url":"https:\/\/afsbio.com\/products\/recombinant-human-lrap-erap2-protein-his-tagactive-pksh031454","provider":"AFSBio Inc.","version":"1.0","type":"link"}