{"product_id":"recombinant-human-map1d-protein-his-tagactive-pksh031376","title":"Recombinant Human MAP1D Protein (His Tag)(Active) - PKSH031376","description":"\u003cp\u003eRecombinant Human MAP1D Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031376-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: MAP1D\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Methionine Aminopeptidase 1D Mitochondrial; Methionyl Aminopeptidase Type 1D Mitochondrial; METAP1D; MAP1D; MAP 1D; MetAP 1D; Metap1l\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; epigenetics and nuclear signal;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_954697.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Methionine aminopeptidase 1D, also known as MAP1D, is a member of thepeptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independant growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC, R\u0026amp;D Systems, Catalog#ES017.The resulting GP-AMC is cleaved by human DPPIV\/CD26, R\u0026amp;D Systems, Catalog#1180SE. The specific activity is \u0026gt; 30 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Arg 44-Ala 335\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 92 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 33.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 36 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104309969075,"sku":"PKSH031376-50","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_fdd7df5b-df2d-4635-bfa0-a981d16df9a1.png?v=1709294003","url":"https:\/\/afsbio.com\/products\/recombinant-human-map1d-protein-his-tagactive-pksh031376","provider":"AFSBio Inc.","version":"1.0","type":"link"}