{"product_id":"recombinant-human-mapkapk5-protein-his-gst-tag-pksh030328","title":"Recombinant Human MAPKAPK5 Protein (His \u0026 GST Tag) - PKSH030328","description":"\u003cp\u003eRecombinant Human MAPKAPK5 Protein (His \u0026amp; GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030328-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: MAPKAPK5\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: MAPKAP-K5, MK-5, MK5, PRAK\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cancer\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_003659.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser\/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1\/ERK, MAPK14\/p38-alpha, and MAPK11\/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Gln 471\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 76 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 82 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 75 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43155021562035,"sku":"PKSH030328-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_ffc432bd-74a3-4560-9619-11d33e35d032.png?v=1710436693","url":"https:\/\/afsbio.com\/products\/recombinant-human-mapkapk5-protein-his-gst-tag-pksh030328","provider":"AFSBio Inc.","version":"1.0","type":"link"}