{"product_id":"recombinant-human-mature-tgf-beta-1-protein-rp01458","title":"Recombinant Human Mature TGF-beta 1 Protein - RP01458","description":"\u003cp\u003eRecombinant Human Mature TGF-beta 1 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01458-10, RP01458-20, RP01458-50, RP01458-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Mature TGF-beta 1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala279-Ser390) of human Mature TGF-beta 1 (Accession #NP_000651.3) fused with no tag.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, transforming growth factor beta-1, TGF-beta 1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-β\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P01137\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 7040\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-beta 1 at 2 μg\/mL (100 μL\/well) can bind Human TGFBR2 with a linear range of 0.78-11 ng\/mL.|2.Measured by its ability to inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 12.85-51.40 pg\/mL, corresponding to a specific activity of 1.95×10^7~7.78×10^7 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: No tag\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of 50 mM Glycine,150 mM NaCl,pH3.5.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198310473907,"sku":"RP01458-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198310506675,"sku":"RP01458-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198310539443,"sku":"RP01458-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198310572211,"sku":"RP01458-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_28d33a36-5fe8-407d-82af-35fabe32270b.png?v=1766960452","url":"https:\/\/afsbio.com\/products\/recombinant-human-mature-tgf-beta-1-protein-rp01458","provider":"AFSBio Inc.","version":"1.0","type":"link"}