{"product_id":"recombinant-human-mfg-e8-lactadherin-mfge8-protein-his-tagactive-pksh031387","title":"Recombinant Human MFG-E8\/lactadherin\/MFGE8 Protein (His Tag)(Active) - PKSH031387","description":"Recombinant Human MFG-E8\/lactadherin\/MFGE8 Protein (His Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH031387-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: MFG-E8; lactadherin; MFGE8\n\nTarget Symptom: BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8; MFGM; OAcGD3S; SED1; SPAG10\n\nTarget Species: Human\n\nExpression Host: Baculovirus-Insect Cells\n\nApplication: Cell culture\n\nFusion Tag: C-His\n\nUNIProt ID: Q08431\n\nAccession: Q08431-1\n\nBackground: MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5\/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.\n\nActivity: When 5 x 10\u003csup\u003e4\u003c\/sup\u003e cells\/well are added to Recombinant Human MFG-E8 coated plates (12.5 μg\/mL, 100 μL\/well), 45-85% cells will adhere after 1 hour at 37℃.\n\nSequence: Met 1-Cys 387\n\nPurity: \u0026gt; 80 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS，0.2M Arg，0.01% Tween-20, pH7.5.\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 42 kDa\n\nObserved MW: 45 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104352239795,"sku":"PKSH031387-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_f2fac7d6-93ef-4653-89c7-bebb7c6a88a2.png?v=1709294987","url":"https:\/\/afsbio.com\/products\/recombinant-human-mfg-e8-lactadherin-mfge8-protein-his-tagactive-pksh031387","provider":"AFSBio Inc.","version":"1.0","type":"link"}