{"product_id":"recombinant-human-mica-protein-fc-tagactive-pksh032753","title":"Recombinant Human MICA Protein (Fc Tag)(Active) - PKSH032753","description":"\u003cp\u003eRecombinant Human MICA Protein (Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032753-10, PKSH032753-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: MICA\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: MHC Class I Polypeptide-Related Sequence A; MIC-A; MICA; PERB11.1\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-Fc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q29983\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q29983\u003c\/p\u003e\n\n\u003cp\u003eBackground: MHC class I polypeptide-related sequence A, also known as MIC-A, PERB11.1 and MICA, is a single-pass type I membrane protein which belongs to the MHC class I family of MIC subfamily. MICA contains one Ig-like C1-type domain and is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA is the ligand for NK cell activating receptor KLRK1\/NKG2D. MICA seems to have no role in antigen presentation. MICA leads to cell lysis by binding to KLRK1.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Human MICA-Fc at 2μg\/ml(100 μl\/well) can bind Human NKG2DL2-His(Cat: PKSH032816). The ED50 of Human MICA-Fc is 2. 29 ug\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala23-Glu308\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 59.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 88 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118148780211,"sku":"PKSH032753-10","price":163.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118148812979,"sku":"PKSH032753-50","price":415.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_9ffe066d-98f7-4679-bfa2-d7595917f74d.png?v=1709637107","url":"https:\/\/afsbio.com\/products\/recombinant-human-mica-protein-fc-tagactive-pksh032753","provider":"AFSBio Inc.","version":"1.0","type":"link"}